Optimizing the downstream MVA pathway using a combination optimization strategy to increase lycopene yield in Escherichia coli.

ABSTRACT:

Background

Lycopene is increasing in demand due to its widespread use in the pharmaceutical and food industries. Metabolic engineering and synthetic biology technologies have been widely used to overexpress the heterologous mevalonate pathway and lycopene pathway in Escherichia coli to produce lycopene. However, due to the tedious metabolic pathways and complicated metabolic background, optimizing the lycopene synthetic pathway using reasonable design approaches becomes difficult.

Results

In this study, the heterologous lycopene metabolic pathway was introduced into E. coli and divided into three modules, with mevalonate and DMAPP serving as connecting nodes. The module containing the genes (MVK, PMK, MVD, IDI) of downstream MVA pathway was adjusted by altering the expressio

SUBMITTER: Cheng T 

PROVIDER: S-EPMC9208136 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature