KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation

ABSTRACT:

Background

The microtubule-dependent molecular motor protein Kinesin Family Member 2A (KIF2A) is down-regulated in asthmatic human airway epithelium. However, little is known about the roles of KIF2A as well as the possible underlying mechanisms in asthma.

Methods

House dust mite (HDM) extract was administered to establish a murine model of asthma. The expression of KIF2A, IL-33 and the autophagy pathways were detected. The plasmid pCMV-KIF2A was used to overexpress KIF2A in the airway epithelial cells in vitro and in vivo. IL-4, IL-5, IL-33 and other cytokines in bronchoalveolar lavage fluid (BALF) and lung tissues homogenates were measured.

Results

In response to the challenge of house dust mite (HDM) in vitro and in vivo, airway epithelial cells displayed decr

SUBMITTER: Wang Z 

PROVIDER: S-EPMC9208156 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature