Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality.

ABSTRACT:

Background

Ovarian follicular fluids (FFs) contain several kinds of regulatory factors that maintain a suitable microenvironment for oocyte development. Extracellular vesicles (EVs) are among the factors that play essential roles in regulating follicle and oocyte development through their cargo molecules that include microRNAs (miRNAs). This study aimed to investigate small-EV (s-EV) miRNAs in porcine FFs and their potential association with oocyte quality.

Methods

Individual aspirated oocytes were stained with lissamine green B stain (LB), a vital stain for oocyte quality, and each oocyte was classified as high-quality (unstained; HQ) or low-quality (stained; LQ). FFs corresponding to oocytes were pooled together into HQ and LQ groups. Small-EVs were isolated from FFs, char

SUBMITTER: Gad A 

PROVIDER: S-EPMC9208166 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature