Complement C3a activates astrocytes to promote medulloblastoma progression through TNF-α.

ABSTRACT:

Background

Medulloblastoma (MB) is the most common malignant brain tumor in children. Approximately one-third of MB patients remain incurable. Understanding the molecular mechanism of MB tumorigenesis is, therefore, critical for developing specific and effective treatment strategies. Our previous work demonstrated that astrocytes constitute the tumor microenvironment (TME) of MB and play an indispensable role in MB progression. However, the underlying mechanisms by which astrocytes are regulated and activated to promote MB remain elusive.

Methods

By taking advantage of Math1-Cre/Ptch1^loxp/loxp mice, which spontaneously develop MB, primary MB cells and astrocytes were isolated and then subjected to administration and coculture in vitro. Immunohistochemistry was uti

SUBMITTER: Gong B 

PROVIDER: S-EPMC9208237 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature