In silico multi-epitope Bunyumwera virus vaccine to target virus nucleocapsid N protein.

ABSTRACT:

Background

Bunyumwera virus can cause 82% mortality in humans currently with no vaccine or drugs for treatment. We described an in silico multi-epitope vaccine targeting Bunyumwera virus nucleocapsid N-protein and predicted B and T cell epitopes for immunogenicity, allergenicity, toxicity, and conservancy. For creating the most potent immunological response possible, docking epitopes with HLA alleles are chosen to screen them. The 3D vaccination was docked with the Toll-like receptor-8 using molecular dynamic simulations. To ensure production efficiency, the vaccine sequence was further cloned in silico in a plasmid pIB2 vector. For efficacy and safety, results must be supported in vitro and in vivo.

Results

The vaccine was cloned to enable expression and translation in a pl

SUBMITTER: Nelluri KDD 

PROVIDER: S-EPMC9208256 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature