Combined Mechanical and Enzymatic Dissociation of Mouse Brain Hippocampal Tissue.

ABSTRACT: This neural dissociation protocol (an adaptation of the protocol accompanying a commercial adult brain dissociation kit) optimizes tissue processing in preparation for detailed downstream analysis such as flow cytometry or single-cell sequencing. Neural dissociation can be conducted via mechanical dissociation (such as using filters, chopping techniques, or pipette trituration), enzymatic digestion, or a combination thereof. The delicate nature of neuronal cells can complicate efforts to obtain the highly viable, true single-cell suspension with minimal cellular debris that is required for single-cell analysis. The data demonstrate that this combination of automated mechanical dissociation and enzymatic digestion consistently yields a highly viable (>90%) single-cell suspension, overcoming

SUBMITTER: Trujillo M 

PROVIDER: S-EPMC9208324 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature