Oxidative stress induces Ser 2 dephosphorylation of the RNA polymerase II CTD and premature transcription termination.

ABSTRACT: The C-terminal domain (CTD) of the largest subunit of RNA polymerase II (Pol II) consists of YSPTSPS heptapeptide repeats, and the phosphorylation status of the repeats controls multiple transcriptional steps and co-transcriptional events. However, how CTD phosphorylation status responds to distinct environmental stresses is not fully understood. In this study, we found that a drastic reduction in phosphorylation of a subset of Ser2 residues occurs rapidly but transiently following exposure to H₂O₂. ChIP analysis indicated that Ser2-P, and to a lesser extent Tyr1-P was reduced only at the gene 3' end. Significantly, the levels of polyadenylation factor CstF77, as well as Pol II, were also reduced. However, no increase in uncleaved or readthrough RNA products was obser

SUBMITTER: Yamazaki T 

PROVIDER: S-EPMC9208776 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature