Global stable-isotope tracing metabolomics reveals system-wide metabolic alternations in aging Drosophila.

ABSTRACT: System-wide metabolic homeostasis is crucial for maintaining physiological functions of living organisms. Stable-isotope tracing metabolomics allows to unravel metabolic activity quantitatively by measuring the isotopically labeled metabolites, but has been largely restricted by coverage. Delineating system-wide metabolic homeostasis at the whole-organism level remains challenging. Here, we develop a global isotope tracing metabolomics technology to measure labeled metabolites with a metabolome-wide coverage. Using Drosophila as an aging model organism, we probe the in vivo tracing kinetics with quantitative information on labeling patterns, extents and rates on a metabolome-wide scale. We curate a system-wide metabolic network to characterize metabolic homeostasis and disclose a system-wi

SUBMITTER: Wang R 

PROVIDER: S-EPMC9209425 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature