A colorimetric method to measure in vitro nitrogenase functionality for engineering nitrogen fixation.

ABSTRACT: Biological nitrogen fixation (BNF) is the reduction of N₂ into NH₃ in a group of prokaryotes by an extremely O₂-sensitive protein complex called nitrogenase. Transfer of the BNF pathway directly into plants, rather than by association with microorganisms, could generate crops that are less dependent on synthetic nitrogen fertilizers and increase agricultural productivity and sustainability. In the laboratory, nitrogenase activity is commonly determined by measuring ethylene produced from the nitrogenase-dependent reduction of acetylene (ARA) using a gas chromatograph. The ARA is not well suited for analysis of large sample sets nor easily adapted to automated robotic determination of nitrogenase activities. Here, we show that a reduced sulfonated viologen d

SUBMITTER: Paya-Tormo L 

PROVIDER: S-EPMC9209457 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature