A clinically annotated post-mortem approach to study multi-organ somatic mutational clonality in normal tissues.

ABSTRACT: Recent research on normal human tissues identified omnipresent clones of cells, driven by somatic mutations known to be responsible for carcinogenesis (e.g., in TP53 or NOTCH1). These new insights are fundamentally changing current tumor evolution models, with broad oncological implications. Most studies are based on surgical remnant tissues, which are not available for many organs and rarely in a pan-organ setting (multiple organs from the same individual). Here, we describe an approach based on clinically annotated post-mortem tissues, derived from whole-body donors that are routinely used for educational purposes at human anatomy units. We validated this post-mortem approach using UV-exposed and unexposed epidermal skin tissues and confirm the presence of positively selected NOTCH1/2-,

SUBMITTER: Luijts T 

PROVIDER: S-EPMC9209481 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature