Adipose cells and tissues soften with lipid accumulation while in diabetes adipose tissue stiffens.

ABSTRACT: Adipose tissue expansion involves both differentiation of new precursors and size increase of mature adipocytes. While the two processes are well balanced in healthy tissues, obesity and diabetes type II are associated with abnormally enlarged adipocytes and excess lipid accumulation. Previous studies suggested a link between cell stiffness, volume and stem cell differentiation, although in the context of preadipocytes, there have been contradictory results regarding stiffness changes with differentiation. Thus, we set out to quantitatively monitor adipocyte shape and size changes with differentiation and lipid accumulation. We quantified by optical diffraction tomography that differentiating preadipocytes increased their volumes drastically. Atomic force microscopy (AFM)-indentation and -

SUBMITTER: Abuhattum S 

PROVIDER: S-EPMC9209483 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature