The profiling and analysis of gene expression in human periodontal ligament tissue and fibroblasts.

ABSTRACT:

Objectives

The periodontal ligament (PDL) is an important component of periodontium to support dental structure in the alveolar socket. Regeneration of PDL tissue is an effective treatment option for periodontal disease and the profiling of genes involved in this process will be informative. Therefore, our study aims to accurately delineate the profiling of gene expression for PDL tissue regeneration.

Materials and methods

We isolated PDL tissues and PDL fibroblasts (PDLFs) from premolar teeth, which were extracted from healthy periodontal status patients undergoing orthodontic treatment. Messenger RNA (mRNA) expression in PDL tissue and PDLFs were analyzed using Cap analysis gene expression, which is a second-generation sequencing technique to create profiling. We also dete

SUBMITTER: Hosiriluck N 

PROVIDER: S-EPMC9209801 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature