3D printed microfluidic device for online detection of neurochemical changes with high temporal resolution in human brain microdialysate.

ABSTRACT: This paper presents the design, optimisation and fabrication of a mechanically robust 3D printed microfluidic device for the high time resolution online analysis of biomarkers in a microdialysate stream at microlitre per minute flow rates. The device consists of a microfluidic channel with secure low volume connections that easily integrates electrochemical biosensors for biomarkers such as glutamate, glucose and lactate. The optimisation process of the microfluidic channel fabrication, including for different types of 3D printer, is explained and the resulting improvement in sensor response time is quantified. The time resolution of the device is characterised by recording short lactate concentration pulses. The device is employed to record simultaneous glutamate, glucose and lactate conc

SUBMITTER: Samper IC 

PROVIDER: S-EPMC9209945 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature