Cloning of the malic enzyme gene from Corynebacterium glutamicum and role of the enzyme in lactate metabolism.

ABSTRACT: Malic enzyme is one of at least five enzymes, known to be present in Corynebacterium glutamicum, capable of carboxylation and decarboxylation reactions coupling glycolysis and the tricarboxylic acid cycle. To date, no information is available concerning the physiological role of the malic enzyme in this bacterium. The malE gene from C. glutamicum has been cloned and sequenced. The protein encoded by this gene has been purified to homogeneity, and the biochemical properties have been established. Biochemical characteristics indicate a decarboxylation role linked to NADPH generation. Strains of C. glutamicum in which the malE gene had been disrupted or overexpressed showed no detectable phenotype during growth on either acetate or glucose, but showed a significant modification of growth beha

SUBMITTER: Gourdon P 

PROVIDER: S-EPMC92100 | biostudies-literature | 2000 Jul

REPOSITORIES: biostudies-literature