Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish.

ABSTRACT: Morphogenesis, the formation of three-dimensional organ structures, requires precise coupling of genetic regulation and complex cell behaviors. The genetic networks governing many morphogenetic systems, including that of the embryonic eye, are poorly understood. In zebrafish, several forward genetic screens have sought to identify factors regulating eye development. These screens often look for eye defects at stages after the optic cup is formed and when retinal neurogenesis is under way. This approach can make it difficult to identify mutants specific for morphogenesis, as opposed to neurogenesis. To this end, we carried out a forward genetic, small-scale haploid mutagenesis screen in zebrafish (Danio rerio) to identify factors that govern optic cup morphogenesis. We screened ∼100 genomes

SUBMITTER: Casey MA 

PROVIDER: S-EPMC9210284 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature