Next-generation sequencing-based bulked segregant analysis without sequencing the parental genomes.

ABSTRACT: Genomic regions that control traits of interest can be rapidly identified using BSA-Seq, a technology in which next-generation sequencing is applied to bulked segregant analysis (BSA). We recently developed the significant structural variant method for BSA-Seq data analysis that exhibits higher detection power than standard BSA-Seq analysis methods. Our original algorithm was developed to analyze BSA-Seq data in which genome sequences of one parent served as the reference sequences in genotype calling and, thus, required the availability of high-quality assembled parental genome sequences. Here, we modified the original script to effectively detect the genomic region-trait associations using only bulk genome sequences. We analyzed two public BSA-Seq datasets using our modified method and t

SUBMITTER: Zhang J 

PROVIDER: S-EPMC9210294 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature