Generation of immunocompetent syngeneic allograft mouse models for pediatric diffuse midline glioma.

ABSTRACT:

Background

Diffuse midline gliomas (DMG) are highly malignant incurable pediatric brain tumors. A lack of effective treatment options highlights the need to investigate novel therapeutic strategies. This includes the use of immunotherapy, which has shown promise in other hard-to-treat tumors. To facilitate preclinical immunotherapeutic research, immunocompetent mouse models that accurately reflect the unique genetic, anatomical, and histological features of DMG patients are warranted.

Methods

We established cell cultures from primary DMG mouse models (C57BL/6) that were generated by brainstem targeted intra-uterine electroporation (IUE). We subsequently created allograft DMG mouse models by orthotopically implanting these tumor cells into syngeneic mice. Immunohistochemistry

SUBMITTER: du Chatinier A 

PROVIDER: S-EPMC9210310 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature