Investigation of proteomic profiles in canine lymphoma using tandem mass tag-based quantitative proteomics approach.

ABSTRACT:

Background and aim

Specific tumor biomarkers are useful for the early diagnosis of cancer or can predict the recurrence of neoplastic disease in humans and animals. Lymphoma in dogs could be classified into B-, T-, and NK-cell origins. T-cell lymphoma has the worst prognosis with a shorter survival time and disease-free interval. This study aimed to identify the differential serum protein expressions of canine B- and T-cell lymphomas compared with healthy dogs using a tandem mass tag (TMT)-based quantitative proteomics.

Materials and methods

Serum samples were collected from 20 untreated canine lymphomas (14 B-cells and 6 T-cells) and four healthy control dogs. Sera peptides from each sample were processed for TMT 10-plex tagging and analyzed using liquid chromatography-mass

SUBMITTER: Fonghem P 

PROVIDER: S-EPMC9210836 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature