Arabidopsis thaliana CYCLIC NUCLEOTIDE-GATED CHANNEL2 mediates extracellular ATP signal transduction in root epidermis.

ABSTRACT: Damage can be signalled by extracellular ATP (eATP) using plasma membrane (PM) receptors to effect cytosolic free calcium ion ([Ca²⁺ ]_cyt ) increase as a second messenger. The downstream PM Ca²⁺ channels remain enigmatic. Here, the Arabidopsis thaliana Ca²⁺ channel subunit CYCLIC NUCLEOTIDE-GATED CHANNEL2 (CNGC2) was identified as a critical component linking eATP receptors to downstream [Ca²⁺ ]_cyt signalling in roots. Extracellular ATP-induced changes in single epidermal cell PM voltage and conductance were measured electrophysiologically, changes in root [Ca²⁺ ]_cyt were measured with aequorin, and root transcriptional changes were determined by quantitative real-time PCR. Two cngc2 loss-of-function mu

SUBMITTER: Wang L 

PROVIDER: S-EPMC9211375 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature