Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli.

ABSTRACT: Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

SUBMITTER: Henne A 

PROVIDER: S-EPMC92121 | biostudies-literature | 2000 Jul

REPOSITORIES: biostudies-literature