High throughput SARS-CoV-2 variant analysis using molecular barcodes coupled with next generation sequencing.

ABSTRACT: The identification of SARS-CoV-2 variants across the globe and their implications on the outspread of the pandemic, infection potential and resistance to vaccination, requires modification of the current diagnostic methods to map out viral mutations rapidly and reliably. Here, we demonstrate that integrating DNA barcoding technology, sample pooling and Next Generation Sequencing (NGS) provide an applicable solution for large-population viral screening combined with specific variant analysis. Our solution allows high throughput testing by barcoding each sample, followed by pooling of test samples using a multi-step procedure. First, patient-specific barcodes are added to the primers used in a one-step RT-PCR reaction, amplifying three different viral genes and one human housekeeping gene (a

SUBMITTER: Cohen-Aharonov LA 

PROVIDER: S-EPMC9212143 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature