Identifying enhancers of innate immune signaling as broad-spectrum antivirals active against emerging viruses.

ABSTRACT: The increasingly frequent outbreaks of pathogenic viruses have underlined the urgent need to improve our arsenal of antivirals that can be deployed for future pandemics. Innate immunity is a powerful first line of defense against pathogens, and compounds that boost the innate response have high potential to act as broad-spectrum antivirals. Here, we harnessed localization-dependent protein-complementation assays (called Alpha Centauri) to measure the nuclear translocation of interferon regulatory factors (IRFs), thus providing a readout of innate immune activation following viral infection that is applicable to high-throughput screening of immunomodulatory molecules. As proof of concept, we screened a library of kinase inhibitors on severe acute respiratory syndrome coronavirus 2 (SARS-CoV

SUBMITTER: Maarifi G 

PROVIDER: S-EPMC9213012 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature