Deletion of Mettl3 at the Pro-B Stage Marginally Affects B Cell Development and Profibrogenic Activity of B Cells in Liver Fibrosis.

ABSTRACT: N6-methyladenosine (m⁶A) modification plays a pivotal role in cell fate determination. Previous studies show that eliminating m⁶A using Mb1-Cre dramatically impairs B cell development. However, whether disturbing m⁶A modification at later stages affects B cell development and function remains elusive. Here, we deleted m⁶A methyltransferase Mettl3 from the pro-B stage on using Cd19-Cre (Mettl3 cKO) and found that the frequency of total B cells in peripheral blood, peritoneal cavity, and liver is comparable between Mettl3 cKO mice and wild-type (WT) littermates, while the percentage of whole splenic B cells slightly increases in Mettl3 cKO individuals. The proportion of pre-pro-B, pro-B, pre-B, immature, and matur

SUBMITTER: Kang X 

PROVIDER: S-EPMC9213183 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature