Using fluorescent promoter-reporters to study sugar utilization control in Bifidobacterium longum NCC 2705.

ABSTRACT: Bifidobacteria are amongst the first bacteria to colonize the human gastro-intestinal system and have been proposed to play a crucial role in the development of the infant gut since their absence is correlated to the development of diseases later in life. Bifidobacteria have the capacity to metabolize a diverse range of (complex) carbohydrates, reflecting their adaptation to the lower gastro-intestinal tract. Detailed understanding of carbohydrate metabolism regulation in this genus is of prime importance and availability of additional genetic tools easing such studies would be beneficial. To develop a fluorescent protein-based reporter system that can be used in B. longum NCC 2705, we first selected the most promising fluorescent protein out of the seven we tested (i.e., mCherry). This re

SUBMITTER: Duboux S 

PROVIDER: S-EPMC9213400 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature