Linking metabolites in eight bioactive forage species to their in vitro methane reduction potential across several cultivars and harvests.

ABSTRACT: An in vitro Hohenheim gas test was conducted to analyze the fermentation end-products from 17 cultivars of eight polyphenol containing forage species. The polyphenol composition and proanthocyanidin (PA) structural features of all the cultivars were analyzed with UPLC-MS/MS in leaves of vegetative or generative plants. The samples were incubated with and without polyethylene glycol (PEG, a tannin-binding agent) to separate the tannin-effect on methane (CH_4, ml/200 mg DM) production from that of forage quality. Sulla and big trefoil, two particularly PA rich species, were found to have the highest CH₄ reduction potential of up to 47% when compared to the samples without PEG. However, concomitant reduction in gas production (GP, ml/200 mg DM) of up to 44% was also obser

SUBMITTER: Verma S 

PROVIDER: S-EPMC9213545 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature