Novel immortalization approach defers senescence of cultured canine adipose-derived mesenchymal stromal cells.

ABSTRACT: In the last decades, the scientific community spared no effort to elucidate the therapeutic potential of mesenchymal stromal cells (MSCs). Unfortunately, in vitro cellular senescence occurring along with a loss of proliferative capacity is a major drawback in view of future therapeutic applications of these cells in the field of regenerative medicine. Even though insight into the mechanisms of replicative senescence in human medicine has evolved dramatically, knowledge about replicative senescence of canine MSCs is still scarce. Thus, we developed a high-content analysis workflow to simultaneously investigate three important characteristics of senescence in canine adipose-derived MSCs (cAD-MSCs): morphological changes, activation of the cell cycle arrest machinery, and increased activity o

SUBMITTER: Stojiljkovic A 

PROVIDER: S-EPMC9213611 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature