Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs.

ABSTRACT: The origin, composition, distribution, and function of cells in the human intervertebral disc (IVD) have not been fully understood. Here, cell atlases of both human neonatal and adult IVDs have been generated and further assessed by gene ontology pathway enrichment, pseudo-time trajectory, histology, and immunofluorescence. Comparison of cell atlases revealed the presence of two subpopulations of notochordal cells (NCs) and their associated markers in both the neonatal and adult IVDs. Developmental trajectories predicted 7 different cell states that describe the developmental process from neonatal to adult cells in IVD and analyzed the NC's role in the IVD development. A high heterogeneity and gradual transition of annulus fibrosus cells (AFCs) in the neonatal IVD was detected and their po

SUBMITTER: Jiang W 

PROVIDER: S-EPMC9213722 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature