DNA damage to β cells in culture recapitulates features of senescent β cells that accumulate in type 1 diabetes.

ABSTRACT:

Objective

Type 1 Diabetes (T1D) is characterized by progressive loss of insulin-producing pancreatic β cells as a result of autoimmune destruction. In addition to β cell death, recent work has shown that subpopulations of β cells acquire dysfunction during T1D. We previously reported that β cells undergoing a DNA damage response (DDR) and senescence accumulate during the pathogenesis of T1D. However, the question of how senescence develops in β cells has not been investigated.

Methods

Here, we tested the hypothesis that unrepaired DNA damage in the context of genetic susceptibility triggers β cell senescence using culture models including the mouse NIT1 β cell line derived from the T1D-susceptible nonobese diabetic (NOD) strain, human donor islets and EndoC β cells. DNA dama

SUBMITTER: Brawerman G 

PROVIDER: S-EPMC9213768 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature