Ontology highlight
ABSTRACT:
SUBMITTER: Breau KA
PROVIDER: S-EPMC9213823 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Breau Keith A KA Ok Meryem T MT Gomez-Martinez Ismael I Burclaff Joseph J Kohn Nathan P NP Magness Scott T ST
Stem cell reports 20220505 6
Two-dimensional (2D) cultures of intestinal and colonic epithelium can be generated using human intestinal stem cells (hISCs) derived from primary tissue sources. These 2D cultures are emerging as attractive and versatile alternatives to three-dimensional organoid cultures; however, transgenesis and gene-editing approaches have not been developed for hISCs grown as 2D monolayers. Using 2D cultured hISCs we show that electroporation achieves up to 80% transfection in hISCs from six anatomical reg ...[more]