Molecular profiling of stem cell-derived retinal pigment epithelial cell differentiation established for clinical translation.

ABSTRACT: Human embryonic stem cell-derived retinal pigment epithelial cells (hESC-RPE) are a promising cell source to treat age-related macular degeneration (AMD). Despite several ongoing clinical studies, a detailed mapping of transient cellular states during in vitro differentiation has not been performed. Here, we conduct single-cell transcriptomic profiling of an hESC-RPE differentiation protocol that has been developed for clinical use. Differentiation progressed through a culture diversification recapitulating early embryonic development, whereby cells rapidly acquired a rostral embryo patterning signature before converging toward the RPE lineage. At intermediate steps, we identified and examined the potency of an NCAM1⁺ retinal progenitor population and showed the ability of the p

SUBMITTER: Petrus-Reurer S 

PROVIDER: S-EPMC9214069 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature