A genetic mammalian proportional-integral feedback control circuit for robust and precise gene regulation.

ABSTRACT: The processes that keep a cell alive are constantly challenged by unpredictable changes in its environment. Cells manage to counteract these changes by employing sophisticated regulatory strategies that maintain a steady internal milieu. Recently, the antithetic integral feedback motif has been demonstrated to be a minimal and universal biological regulatory strategy that can guarantee robust perfect adaptation for noisy gene regulatory networks in Escherichia coli. Here, we present a realization of the antithetic integral feedback motif in a synthetic gene circuit in mammalian cells. We show that the motif robustly maintains the expression of a synthetic transcription factor at tunable levels even when it is perturbed by increased degradation or its interaction network structure is

SUBMITTER: Frei T 

PROVIDER: S-EPMC9214505 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature