The M₁ muscarinic receptor is present in situ as a ligand-regulated mixture of monomers and oligomeric complexes.

ABSTRACT: The quaternary organization of rhodopsin-like G protein-coupled receptors in native tissues is unknown. To address this we generated mice in which the M₁ muscarinic acetylcholine receptor was replaced with a C-terminally monomeric enhanced green fluorescent protein (mEGFP)-linked variant. Fluorescence imaging of brain slices demonstrated appropriate regional distribution, and using both anti-M₁ and anti-green fluorescent protein antisera the expressed transgene was detected in both cortex and hippocampus only as the full-length polypeptide. M₁-mEGFP was expressed at levels equal to the M₁ receptor in wild-type mice and was expressed throughout cell bodies and projections in cultured neurons from these animals. Signaling and behavioral studies dem

SUBMITTER: Marsango S 

PROVIDER: S-EPMC9214538 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature