Duration of dry and humidified incubation of single-step embryo culture medium and oxygen tension during sham culture do not alter medium composition.

ABSTRACT:

Background

The extended embryo culture using single-step medium gained popularity in clinical in vitro fertilisation (IVF). However, there are concerns about the degradation of unstable medium components and their negative effects on the developing embryos. Further, dry-incubation can increase osmolality, which can in-turn enhance the concentration of constituents of the media and their stability. Hence, this study was conducted to understand the immediate changes in the culture media constituents in relation to clinically comparable situations such as single-step extended embryo culture and use of dry and humidified-incubation in two-different gaseous conditions.

Methods

Commercially available single-step medium was sham-cultured in droplets under oil in two different condi

SUBMITTER: Cheredath A 

PROVIDER: S-EPMC9214662 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature