Screening and phylogenetic characterization of tick-borne pathogens in a population of dogs and associated ticks in Egypt.

ABSTRACT:

Background

The incidence or recurrence of tick-borne diseases (TBDs) in animals and humans is increasing rapidly worldwide, but there is insufficient information about TBDs infecting dogs in Egypt. Thus, the present study was conducted to screen and genetically identify tick-borne pathogens (TBPs) in dogs and associated ticks by microscopic examination and polymerase chain reaction (PCR).

Methods

In Cairo and Giza governorates, 208 blood samples were collected from dogs of different breeds, ages, and sex. In addition, 1266 dog-associated ticks were collected (546 ticks were used to prepare hemolymph smears, and 720 ticks were kept in 70% ethanol until PCR analysis). PCR was applied to 124 dog blood samples and 144 tick pools prepared from 720 ticks.

Results

All ticks

SUBMITTER: Hegab AA 

PROVIDER: S-EPMC9215074 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature