Parasites and blood-meal hosts of the tsetse fly in Tanzania: a metagenomics study.

ABSTRACT:

Background

Tsetse flies can transmit various Trypanosoma spp. that cause trypanosomiasis in humans, wild animals, and domestic animals. Amplicon deep sequencing of the 12S ribosomal RNA (rRNA) gene can be used to detect mammalian tsetse hosts, and the 18S rRNA gene can be used to detect all associated eukaryotic pathogens, including Trypanosoma spp.

Methods

Tsetse flies were collected from the Serengeti National Park (n = 48), Maswa Game Reserve (n = 42), and Tarangire National Park (n = 49) in Tanzania in 2012-13. Amplicon deep sequencing targeting mammal-specific 12S rRNA and 18S rRNA genes was performed to screen the blood-feeding sources of tsetse flies and eukaryotic parasites in tsetse flies, respectively.

Results

12S rRNA gene deep sequencing revealed that vari

SUBMITTER: Kim JY 

PROVIDER: S-EPMC9215111 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature