Pancreatic Stellate Cells Prolong Ex Vivo Islet Viability and Function and Improve Engraftment.

ABSTRACT: Preserving islet health and function is critical during pretransplant culture to improve islet transplantation outcome and for ex vivo modeling of diabetes for pharmaceutical drug discovery. The limited islet engraftment potential is primarily attributable to loss of extracellular matrix (ECM) support and interaction. Multipotent cells with ECM depositing competency improve islet survival during short coculture period. However, role of pancreatic stellate cells (PSCs) and their ECM support in preserving ex vivo islet physiology remains largely unknown. Here, we report novel cytoprotective effects of culture-adapted porcine PSCs and role of their ECM-mediated intercellular communication on pig, mouse and human islets ex vivo. Using direct-contact coculture system, we demonstrate that porcin

SUBMITTER: Paul PK 

PROVIDER: S-EPMC9216495 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature