Particle retracking algorithm capable of quantifying large, local matrix deformation for traction force microscopy.

ABSTRACT: Deformation measurement is a key process in traction force microscopy (TFM). Conventionally, particle image velocimetry (PIV) or correlation-based particle tracking velocimetry (cPTV) have been used for such a purpose. Using simulated bead images, we show that those methods fail to capture large displacement vectors and that it is due to a poor cross-correlation. Here, to redeem the potential large vectors, we propose a two-step deformation tracking algorithm that combines cPTV, which performs better for small displacements than PIV methods, and newly-designed retracking algorithm that exploits statistically confident vectors from the initial cPTV to guide the selection of correlation peak which are not necessarily the global maximum. As a result, the new method, named 'cPTV-Retracking', o

SUBMITTER: Haarman SE 

PROVIDER: S-EPMC9216574 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature