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Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022.

ABSTRACT: Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.

SUBMITTER: Claro IM 

PROVIDER: S-EPMC9217064 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022.

Claro Ingra Morales IM   Romano Camila Malta CM   Candido Darlan da Silva DDS   Lima Evelyn Lepka de EL   Lindoso José Angelo Lauletta JAL   Ramundo Mariana Severo MS   Moreira Filipe Romero Rebello FRR   Barra Luiz Alberto Costa LAC   Borges Luciana Marques Sansão LMS   Medeiros Lucas Alberto LA   Tomishige Marcia Y S MYS   Moutinho Tomas T   Silva Anderson José Dias da AJDD   Rodrigues Camila Cristina Martini CCM   Azevedo Luiz Cesar Fernandes de LCF   Villas-Boas Lucy Santos LS   Silva Camila Alves Maia da CAMD   Coletti Thaís Moura TM   Manuli Erika R ER   O'Toole Aine A   Quick Joshua J   Loman Nicholas N   Rambaut Andrew A   Faria Nuno R NR   Figueiredo-Mello Claudia C   Sabino Ester Cerdeira EC  

Revista do Instituto de Medicina Tropical de Sao Paulo 20220624

Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation. ...[more]

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