Cohesin-mediated loop anchors confine the locations of human replication origins.

ABSTRACT: DNA replication occurs through an intricately regulated series of molecular events and is fundamental for genome stability^1,2. At present, it is unknown how the locations of replication origins are determined in the human genome. Here we dissect the role of topologically associating domains (TADs)^3-6, subTADs⁷ and loops⁸ in the positioning of replication initiation zones (IZs). We stratify TADs and subTADs by the presence of corner-dots indicative of loops and the orientation of CTCF motifs. We find that high-efficiency, early replicating IZs localize to boundaries between adjacent corner-dot TADs anchored by high-density arrays of divergently and convergently oriented CTCF motifs. By contrast, low-efficiency IZs localize to weaker dotless bounda

SUBMITTER: Emerson DJ 

PROVIDER: S-EPMC9217744 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature