Cell Morphology as an In Vivo Parameter for the Diagnosis of Limbal Stem Cell Deficiency.

ABSTRACT:

Purpose

The aim of this study was to investigate basal epithelial cell morphology (CM) in the central cornea and limbal areas of eyes with limbal stem cell deficiency (LSCD).

Methods

This was a prospective, cross-sectional comparative study. We developed a CM scoring system based on basal epithelial cell phenotypes graded from 0 (normal) to 3 (severe morphologic alterations); this system was evaluated by 2 independent masked observers. The CM score was compared with the LSCD clinical score, mean best-corrected visual acuity, and in vivo laser scanning confocal microscopy parameters used to stage LSCD (ie, basal epithelial cell density, basal epithelial thickness, and subbasal corneal nerve fiber length density).

Results

One hundred sixty-eight eyes with LSCD and 63 no

SUBMITTER: Bonnet C 

PROVIDER: S-EPMC9218010 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature