PEG₂₀₀₀-DBCO surface coating increases intracellular uptake of liposomes by breast cancer xenografts.

ABSTRACT: Given our interest in the utility of liposomes for molecular imaging and theranostics, we investigated how coating the outer layer of the liposome affects internalization by breast cancer cell lines in vitro and in breast tumor tissues in vivo. Indeed, we discovered that a remarkably high liposomal uptake can be achieved by DBCO (dibenzocyclooctyne) soft coating. Our data demonstrates that decorating the terminal lipid with a DBCO moiety at a specific density induces increased tumor uptake in vivo (tumor uptake ~ 50%) compared to conventional undecorated liposome (tumor uptake ~ 20%). In this study, we report improved visualization of breast cancer cells in vivo using a 4T1 orthotopic breast cancer model and primary breast tumor xenograft models MDA-MB-231 and MDA-MB-436. L-PEG₂₀₀₀

SUBMITTER: Liu D 

PROVIDER: S-EPMC9218082 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature