Identification of G-quadruplex anti-Interleukin-2 aptamer with high specificity through SELEX stringency.

ABSTRACT: Aptamers are short single-stranded oligonucleotides capable of binding to various targets with high specificity and affinity. This study aimed to identify an aptamer against mouse interleukin-2 (mIL-2) as one of the most important cytokines in autoimmune diseases for diagnostic and therapeutic purposes. For this purpose, 14 SELEX rounds were performed on recombinant mIL-2 with high stringency. The dot blot and flow cytometry techniques were conducted to determine affinity, dissociation constant (K_d), specificity, and SELEX rounds screening. The stringency of rounds was considered based on aptamer/target incubation time, washing steps, and target proteins. Finally, the aptamer's structure was mapped and predicted by M-fold and QGRS Mapper web-based software. After 14 rounds, the

SUBMITTER: Momeni M 

PROVIDER: S-EPMC9218155 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature