PACT establishes a posttranscriptional brake on mitochondrial biogenesis by promoting the maturation of miR-181c.

ABSTRACT: The double-stranded RNA-dependent protein kinase activating protein (PACT), an RNA-binding protein that is part of the RNA-induced silencing complex, plays a key role in miR-mediated translational repression. Previous studies showed that PACT regulates the expression of various miRs, selects the miR strand to be loaded onto RNA-induced silencing complex, and determines proper miR length. Apart from PACT's role in mediating the antiviral response in immune cells, what PACT does in other cell types is unknown. Strikingly, it has also been shown that cold exposure leads to marked downregulation of PACT protein in mouse brown adipose tissue (BAT), where mitochondrial biogenesis and metabolism play a central role. Here, we show that PACT establishes a posttranscriptional brake on mitochondrial

SUBMITTER: Dogan AE 

PROVIDER: S-EPMC9218515 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature