Dynamic Interplay between Structural Variations and 3D Genome Organization in Pancreatic Cancer.

ABSTRACT: Structural variations (SVs) are the greatest source of variations in the genome and can lead to oncogenesis. However, the identification and interpretation of SVs in human cancer remain technologically challenging. Here, long-read sequencing is first employed to depict the signatures of structural variations in carcinogenesis of human pancreatic ductal epithelium. Then widespread reprogramming of the 3D chromatin architecture is revealed by an in situ Hi-C technique. Integrative analyses indicate that the distribution pattern of SVs among the 3D genome is highly cell-type specific and the bulk remodeling effects of SVs in the chromatin organization partly depend on intercellular genomic heterogeneity. Meanwhile, contact domains tend to minimize these disrupting effects of SVs within local

SUBMITTER: Du Y 

PROVIDER: S-EPMC9218654 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature