Approaches to Improve the Quantitation of Oxytocin in Human Serum by Mass Spectrometry.

ABSTRACT: The neuropeptide oxytocin (OT) regulates several peripheral and central functions and is a molecule of interest in psychiatric diseases such as autism spectrum disorder, schizophrenia, anxiety and depression. The study of OT in human serum samples is however hampered by inconsistent sample preparation and analysis as well as low endogenous blood concentration (1-10 pM). This results in varying reports on OT's blood levels and interpretation of OT's role in different (patho)physiological states. Quantitative mass spectrometry (MS) is a highly promising technology to address this problem but still requires large sample volumes to achieve adequate sensitivity and reliability for the quantitation of compounds at low concentrations. We therefore systematically evaluated sample preparation metho

SUBMITTER: Hering A 

PROVIDER: S-EPMC9218718 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature