The Role of Peroxisome Proliferator-Activated Receptors in PGF_2α-Induced Luteolysis in the Bovine Corpus Luteum.

ABSTRACT: The participation of peroxisome proliferator-activated receptors (PPARs) in ovarian function in cattle is still not fully understood. The aim of this in vitro study was to determine: (i) the immunolocalization, mRNA expression and tissue concentration of PPARα, PPARδ and PPARγ in the bovine corpus luteum (CL) (n = 40) throughout the estrous cycle, and (ii) the involvement of PPAR in PGF_2α-induced processes related to luteolysis. CL (n = 9) explants were cultured in the presence of PPAR antagonists (10^-5 M) in combination with or without PGF_2α receptor antagonist (10^-5 M) and PGF_2α (10^-6 M). The mRNA and protein expression of PPARs was evaluated through qPCR, IHC, and ELISA, respectively. The results showed that

SUBMITTER: Socha BM 

PROVIDER: S-EPMC9219485 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature