Direct Derivatization in Dried Blood Spots for Oxidized and Reduced Glutathione Quantification in Newborns.

ABSTRACT: The glutathione (GSH)-to-glutathione disulfide (GSSG) ratio is an essential node contributing to intracellular redox status. GSH/GSSG determination in whole blood can be accomplished by liquid chromatography-mass spectrometry (LC-MS) after the derivatization of GSH with N-ethylmaleimide (NEM). While this is feasible in a laboratory environment, its application in the clinical scenario is cumbersome and therefore ranges reported in similar populations differ noticeably. In this work, an LC-MS procedure for the determination of GSH and GSSG in dried blood spot (DBS) samples based on direct in situ GSH derivatization with NEM of only 10 µL of blood was developed. This novel method was applied to 73 cord blood samples and 88 residual blood volumes from routine newborn screening performe

SUBMITTER: Ten-Domenech I 

PROVIDER: S-EPMC9219658 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature