Model-Guided Metabolic Rewiring for Gamma-Aminobutyric Acid and Butyrolactam Biosynthesis in Corynebacterium glutamicum ATCC13032

ABSTRACT:

Simple Summary

The fermentative production of desired chemicals from renewable resources is one of the promising biosynthetic routes to replace the petrochemical-based process. Gamma-aminobutyric acid (GABA) can be synthesized from l-glutamic acid and used as a building block for the synthesis of butyrolactam and polyamide 4 (nylon 4). The genome-scale metabolic model can predict the growth ability and metabolic flux distribution by genetic disturbances, which provides a strategy to construct a microbial cell factory for GABA and butyrolactam biosynthesis. Here, we performed model-guided metabolic engineering of Corynebacterium glutamicum ATCC13032 for GABA and butyrolactam fermentation from glucose. The biosynthetic pathways of GABA and butyrolactam were constructed by overexpres

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC9219837 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature