Optical Fiber-Based Recording of Climbing Fiber Ca²⁺ Signals in Freely Behaving Mice.

ABSTRACT: The olivocerebellar circuitry is important to convey both motor and non-motor information from the inferior olive (IO) to the cerebellar cortex. Several methods are currently established to observe the dynamics of the olivocerebellar circuitry, largely by recording the complex spike activity of cerebellar Purkinje cells; however, these techniques can be technically challenging to apply in vivo and are not always possible in freely behaving animals. Here, we developed a method for the direct, accessible, and robust recording of climbing fiber (CF) Ca²⁺ signals based on optical fiber photometry. We first verified the IO stereotactic coordinates and the organization of contralateral CF projections using tracing techniques and then injected Ca²⁺ indicators optimized for a

SUBMITTER: Tang J 

PROVIDER: S-EPMC9220032 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature