Extraction-Free Absolute Quantification of Circulating miRNAs by Chip-Based Digital PCR.

ABSTRACT: Circulating microRNAs (miRNA) have been proposed as specific biomarkers for several diseases. Quantitative Real-Time PCR (RT-qPCR) is the gold standard technique currently used to evaluate miRNAs expression from different sources. In the last few years, digital PCR (dPCR) emerged as a complementary and accurate detection method. When dealing with gene expression, the first and most delicate step is nucleic-acid isolation. However, all currently available protocols for RNA extraction suffer from the variable loss of RNA species due to the chemicals and number of steps involved, from sample lysis to nucleic acid elution. Here, we evaluated a new process for the detection of circulating miRNAs, consisting of sample lysis followed by direct evaluation by dPCR in plasma from healthy donors and

SUBMITTER: D'Alessandra Y 

PROVIDER: S-EPMC9220272 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature